Nombre: GABRIELLA SOARES BORGES SALOMÃO
Tipo: MSc dissertation
Fecha de publicación: 16/03/2017
Supervisor:
Nombre |
Rol |
|---|---|
| LAURA MARINA PINOTTI | Advisor * |
Junta de examinadores:
| Nombre |
Rol |
|---|---|
| IARA REBOUÇAS PINHEIRO | External Examiner * |
| RODRIGO RANDOW DE FREITAS | Co advisor * |
| TAISA SHIMOSAKAI DE LIRA | Internal Examiner * |
Resumen: Cellulases are enzymes capable of degrading cellulose, the most abundant biopolymer in nature. Currently the alcohol industry has been giving, special attention to cellulases for the production process of second-generation ethanol. There are many cellulases producer organisms in nature, especially filamentous fungi. For the production of these enzymes to occur it's necessary cellulosic material in the fermentation medium. It can be provided by complex media, as agroindustrial
wastes, or chemically defined media. When using waste its interesting that material undergoes a pretreatment process with the objective of eliminating other components
that hinder the microbial attack of cellulose, the main source of carbon of the microorganisms for cellulases production. Sugarcane bagasse is a residue of the sugar industry that has been widely studied as substrate for cellulase production.
Therefore, the goal of this work had to study the best operational conditions (temperature, bagasse concentration and humidity content) for cellulases production
from fungi Penicillium sp., Rhizomucor sp. e Trichoderma koningii INCQS 40331 (CFAM 422) natural sugarcane bagasse and pre-treated by acid-alkaline solution as
substrates under solid state and submerged fermentation. For this it will be used a factorial experimental design type (32) with two central points, totaling 11 experiments for each pair microorganism/substrate. The fungi Trichoderma koningii proved the best cellulases producer and as substrate, the natural bagasse led to the best
results. The fermentation in the solid state submerged fermentation presented the best results (8,199 UI/gs CMCase) at 28ºC and 50% humidity (wet basis). Regarding the submerged fermentation the best enzymatic production (3130.43 IU/L
CMCase) was also observed at 28ºC and 2.7% (m/v) of substrate concentration.
