Nombre: RENAN AMORIM MARGON
Tipo: MSc dissertation
Fecha de publicación: 28/03/2018
Supervisor:
Nombre |
Rol |
|---|---|
| LAURA MARINA PINOTTI | Co-advisor * |
| RODRIGO RANDOW DE FREITAS | Advisor * |
Junta de examinadores:
| Nombre |
Rol |
|---|---|
| MÁRCIA VANACOR BARROSO | External Alternate * |
| MARISTELA DE ARAUJO VICENTE | Internal Alternate * |
| RENATO DO NASCIMENTO SIQUEIRA | External Examiner * |
| RODRIGO RANDOW DE FREITAS | Advisor * |
| TAISA SHIMOSAKAI DE LIRA | Internal Examiner * |
Resumen: The search for cleaner and renewable energy sources has been intensifying studies of alternative energies to achieve their economic, technological and environmental viability. In this context, the production of second generation bioethanol has been considered as a way to meet this demand. Besides being able to solve partially the dependence of the use of fossil fuels, this technology stands out by for utilizing forest and industrial lignocellulosic residues, adding value to this material. Several works are being carried out to increase the yield of its production, either with more efficient pre-treatments or with adjustments of parameters, aiming to reach the optimization of the process. Thus, the objective of this work is the study of the influence of selected variables of the enzymatic hydrolysis process as an alternative for the use of residual biomass from the pulp and paper industry. This study aims at the transformation of the cellulose in the material into reducing sugars, which can later be fermented for bioethanol production. The biomass must initially undergo a pretreatment to increase its digestibility and make the cellulose more accessible to the enzymes. In this work, two-stage pretreatment was used, which consists of first treating the material with dilute acid and subsequently using an alkaline solution. The enzymatic hydrolysis was carried out in the eucalyptus in natura and pre-treated. A supplementation of β-glucosidases to the enzymatic cocktail was also made to evaluate its effects on inhibitory substances. The studied variables were: hydrolysis time (24 h, 48 h and 72 h); the enzyme concentration (10, 20 and 30 FPU / g biomass); and the particle size (2.0-1.4, 1.4-0.8, 0.8-0.2 mm). The enzymatic concentration was the parameter that most influenced the yield, followed by the lower particle size. The time had less influence on the result after 24 h. Pretreatment was shown to be essential when removing hemicellulose from 18.53 to 1.91% and lignin from 23.51 to 13.22% m/m. This stage directly reflected in the yield, in which eucalyptus in natura, pretreated and with supplementation of β-glucosidases was 167.59, 729.05 and 1110.42 mols ART / ton biomass and 19.57, 56.76 and 86.45 L theoretical bioethanol / dry eucalypt, respectively.
Keywords: Enzymatic hydrolysis. Pretreatment. Eucalyptus. Bioethanol.
